Journal: Frontiers in Microbiology
Article Title: Dangua Fang induces anti-glucolipid metabolism disorder effects similar to those of direct NFIL3 inhibition
doi: 10.3389/fmicb.2025.1557345
Figure Lengend Snippet: Effects of DGF or NFIL3 inhibition on NFIL3. (A–C) NFIL3 levels in the serum, liver, and small intestine. (D) NFIL3 mRNA levels in the liver. (E,G) NFIL3 protein levels in the liver. (F) Positive area of NFIL3. (H) Immunohistochemistry of small intestine (magnification: ×200). Data are expressed as mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001. N = 6 per group. NFIL3, Nuclear factor, interleukin-3 regulated; CG, Control group; MG, Model group; DFG, Dangua Fang group; DFSG, Dangua Fang + siRNA group; SG, siRNA group.
Article Snippet: 0.9% Sodium chloride injection (Kelun Pharmaceutical Co., Ltd., Nanchang, China, H20083400); siRNA-NFIL3 (Shanghai GenePharma Co., Ltd., Shanghai, China, TS20230428003); ethyl carbamate (Sigma-Aldrich Trading Co., Shanghai, China, PH006652); phosphate-buffered saline (PBS), bicinchoninic acid (BCA) protein assay kit, antibody dilution solution, streptavidin-biotin complex (SABC) immunohistochemistry staining kit, and DAB chromogenic kit (Boster Biological Technology Co., Ltd., Wuhan, China, PYG0072, AR0146, AR1017, SA1020, AR1027); total cholesterol (TC), triglyceride (TG), and free fatty acid (FFA) assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China, A111-2-1, A110-1-1, A042-2-1); mouse NFIL3 enzyme-linked immunosorbent assay (ELISA) kit (Shanghai Westang Bio-Tech, Shanghai, China, FP11773); haematoxylin-eosin (HE) staining solution (Lanjieke Technology Co., Ltd., Beijing, China, BL700B); Masson staining kit and Oil Red O staining solution (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China, G1340, G1261).
Techniques: Inhibition, Immunohistochemistry, Standard Deviation, Control